Team:Kyoto/CiC/Experiment

From 2009.igem.org

(Difference between revisions)
(Evaluation for the performance of Timer Vector)
(Construction)
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===Construction===
===Construction===
HIV-TAT::(LALAAAA)3 expressing vector
HIV-TAT::(LALAAAA)3 expressing vector
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[[Image:HIV-TAT.png]]
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[[Image:HIV-TAT.png|700px|thumb|center|]]
†RBS+HIV-TAT+HIStag+(LALAAAA)3 was made by elongation of primer dimer.
†RBS+HIV-TAT+HIStag+(LALAAAA)3 was made by elongation of primer dimer.
(Forward primer;  
(Forward primer;  
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Signal for TIM23 complex::GFP expressing vector
Signal for TIM23 complex::GFP expressing vector
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[[Image:sigGFP.png]]
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[[Image:sigGFP.png|700px|thumb|center|]]
†RBS+Signal for TIM23 complex::GFP+terminator was made by two-stage PCR.
†RBS+Signal for TIM23 complex::GFP+terminator was made by two-stage PCR.
First-stage PCR
First-stage PCR

Revision as of 05:19, 20 October 2009

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  3. Modelling

Experiment

Construction

HIV-TAT::(LALAAAA)3 expressing vector

HIV-TAT.png

†RBS+HIV-TAT+HIStag+(LALAAAA)3 was made by elongation of primer dimer. (Forward primer; cggaattcgcggccgcttctagagaaagaggagaaatactagATGTATGGACGTAAAAAACGTCGTGGACGTCGTCGTGGCGGCGGTCATCATCATCATCACCATGGCGG Reverse primer; ctgcagcggccgctactagtaTTACGCGGCCGCCGCCAGGGCCAGCGCGGCCGCCGCCAGGGCCAGCGCGGCCGCCGCCAGGGCCAGGCCACCGCCATGGTGATGATGATG)

Signal for TIM23 complex::GFP expressing vector

SigGFP.png

†RBS+Signal for TIM23 complex::GFP+terminator was made by two-stage PCR. First-stage PCR Forward primer; TTTAAACCGGCGACCCGTACCCTGTGCTCTTCTCGTTATCTGCTGcgtaaaggagaagaacttttcactggagttg Reverse primer; agtgagctgataccgctcgc

Second-stage PCR Forward primer; cggaattcgcggccgcttctagagaaagaggagaaatactagATGCTGAGCCTGCGTCAGTCTATTCGTTTTTTTAAACCGGCGACCCGTAC Reverse primer; agtgagctgataccgctcgc