Team:Kyoto/CiC/Results

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(sig-GFP expression in HeLa)
(sig-GFP expression in HeLa)
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===Result of Subgoal C===
===Result of Subgoal C===
====sig-GFP expression in HeLa====
====sig-GFP expression in HeLa====
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We confirmed the function of the signal sequence for importing protein into mitochondria,
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To confirm the function of the signal sequence for importing protein into mitochondria,
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compared the expressioin pattern of sig-GFP or GFP with mitotracker signal.
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we compared the expressioin pattern of sig-GFP or GFP with mitotracker signal.
The GFP signal was detected throughout the cell except for the black granules in the cytoplasm or the nuclear,
The GFP signal was detected throughout the cell except for the black granules in the cytoplasm or the nuclear,
while sig-GFP signal showed the string-like pattern in the cytoplasm. The black granules in the cytoplasm observed
while sig-GFP signal showed the string-like pattern in the cytoplasm. The black granules in the cytoplasm observed
in the GFP-expressing cell were stained by mitotracker, and the result indicated that the GFP was
in the GFP-expressing cell were stained by mitotracker, and the result indicated that the GFP was
normally eliminated from mitochondria (Fig. NUMBER, GFP and mitotracker merged). In case of sig-GFP,
normally eliminated from mitochondria (Fig. NUMBER, GFP and mitotracker merged). In case of sig-GFP,
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mitochondria stained by a mitotracker and the GFP signal showed almost the same pattern and
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mitochondria stained by a mitotracker and the GFP signal showed almost the same pattern.
the yellow color in the merge images (Fig. Number, sig-GFP and mitotracker merged) suggested that
the yellow color in the merge images (Fig. Number, sig-GFP and mitotracker merged) suggested that
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the sig-GFP and mitochondria were co-localize in the HeLa cell.
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the sig-GFP and mitochondria were colocalized in the HeLa cell.
We, consequently, our constructed signal sequence has the function of importing protein into mitochondria as expected.
We, consequently, our constructed signal sequence has the function of importing protein into mitochondria as expected.
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Revision as of 14:39, 21 October 2009

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Results & Discussion

Result of Subgoal A

Result of Subgoal B

Result of Subgoal C

sig-GFP expression in HeLa

To confirm the function of the signal sequence for importing protein into mitochondria, we compared the expressioin pattern of sig-GFP or GFP with mitotracker signal. The GFP signal was detected throughout the cell except for the black granules in the cytoplasm or the nuclear, while sig-GFP signal showed the string-like pattern in the cytoplasm. The black granules in the cytoplasm observed in the GFP-expressing cell were stained by mitotracker, and the result indicated that the GFP was normally eliminated from mitochondria (Fig. NUMBER, GFP and mitotracker merged). In case of sig-GFP, mitochondria stained by a mitotracker and the GFP signal showed almost the same pattern. the yellow color in the merge images (Fig. Number, sig-GFP and mitotracker merged) suggested that the sig-GFP and mitochondria were colocalized in the HeLa cell. We, consequently, our constructed signal sequence has the function of importing protein into mitochondria as expected.