22 July 2009

Sriram

Observed growth in all the 5 culture tubes [BBa_E0040 - GFP, BBa_C0040 - TetR, BBa_C0051 - cl, BBa_I12006 - λp, BBa_E1010 - mRFP1] and at 11 AM the DNA extraction was started and done with miniprep plasmid DNA extraction kit. The DNA concentrations were noted for all the amplified biobrick parts using Nanodrop. [The values must be given below...It is in lab notes ... will be updated tomorrow...]

Agarose gel electrophoresis was run for all the 12 parts amplified with 2µl loading buffer for 20 minutes (17:30 to 17:50)and the gel is stored in fridge to continue with electrophoresis tomorrow.

Daniel

Prepared the glycerol stocks for the remaining culture of the 5 biobricks for future use. The protocol is as follows:

1. Centrifuge 1ml of culture in eppendorf at 8000rpm for 3 minutes at 15°C.
2. Decant the supernatant.
3. Add 1 ml of culture and centrifuge at 8000rpm for 3 minutes at 15°C.
4. Decant the supernatant.
5. Add 1 ml of culture and centrifuge at 8000rpm for 3 minutes at 15°C.
6. Decant the supernatant.
7. Add 1 ml of culture and centrifuge at 8000rpm for 3 minutes at 15°C.
8. Decant 0.5ml of the supernatant.
9. Resuspend the dense pellet throughly, add 0.5 ml of 10% glycerol and store in -80°C freezer.

Calin