• Home
• The Project
  • Motivation
  • Solution
  • Results & Conclusions
  • References
• Materials & Methods
  • Measurements
  • Instruments
  • Protocols
• Software Developed
• Parts
  • Submitted
  • Characterization
• The Team
• Notebook
  • Week-book
  • Freezer Management
• Biological Safety
• Pavia
  • History
  • University
  • Gallery
• Sponsors
• Gallery
• Team Locations

Here we describe the characterization results of 4 parts of our own design, 2 existing parts re-built because they were inconsistent and 7 existing parts taken from the Registry.

Our new parts:

• BBa_K173003 - ethanol producing device
• BBa_K173007 - aTc inducible device with J23100 promoter
• BBa_K173011 - aTc inducible device with J23118 promoter
• BBa_K173010 - lactose/IPTG inducible device with J23118 promoter

Re-built existing parts (BBa_our part code/BBa_existing part code):

• BBa_K173004/BBa_I732019 - beta-galactosidase protein generator
• BBa_K173005/BBa_Q04400 - tetR QPI

Existing parts from the Registry:

• BBa_J23100, BBa_J23101, BBa_J23118 - constitutive promoter family members
• BBa_F2620 - 3OC6HSL receiver device
• BBa_K116001 - nhaA promoter
• BBa_K112808 - Enterobacteria phage T4 Lysis Device
• BBa_R0011 - Plac hybrid promoter

Our new parts

BBa_K173003 - ethanol producing device

BBa_K173007 - aTc inducible device with J23100 promoter

BBa_K173011 - aTc inducible device with J23118 promoter

BBa_K173010 - lactose/IPTG inducible device with J23118 promoter

Re-built existing parts

BBa_K173004/BBa_I732019 - beta-galactosidase protein generator

BBa_K173005/BBa_Q04400 - tetR QPI

Existing parts from the Registry

BBa_J23100, BBa_J23101, BBa_J23118 - constitutive promoter family members

BBa_F2620 - 3OC6HSL receiver device

BBa_K116001 - nhaA promoter

BBa_K112808 - Enterobacteria phage T4 Lysis Device

BBa_R0011 - Plac hybrid promoter

Data analysis

Growth curves

The presented growth curves have all been processed as OD600_culture-OD600_broth for each time sample. OD600_broth is the medium in the same conditions as in the culture (e.g. induced with the same inducer concentration as in the culture).

Relative Promoter Units (RPUs)

The RPUs are standard units proposed by Kelly J. et al., 2008, in which the transcriptional strength of a promoter can be measured using a reference standard, just like the ground in electric circuits.

RPUs have been computed as:

in which:

• phi is the promoter of interest and J23101 is the reference standard promoter (taken from Anderson Promoter Collection);
• F is the blanked fluorescence of the culture, computed as F_culture-F_negative_control for each time sample, where the negative control is a non-fluorescent strain;
• ABS is the blanked absorbance (OD600) of the culture, computed as described in "Growth curves" section.

RPU measurement has the following advantages:

• it is proportional to PoPS (Polymerase Per

of a reporter protein with a high half life (in our case GFPmut3, , which has an estimated half life of at least 24 hours);