Team:Valencia/WetLab

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== '''Yeast Team''' ==
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<html><a href="https://2009.igem.org/Team:Valencia/WetLab/YeastTeam" target="_top"> <img src="https://static.igem.org/mediawiki/2009/e/e8/Yeastteam.jpg" width="300" height="230"></a></html>
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An important part of our project is to work with '''yeasts''' in order to create each and everyone of the cell-pixels of our bioscreen. We will use some aequorin-transformed yeast strains.
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[https://2009.igem.org/Team:Valencia/TeamVal https://static.igem.org/mediawiki/2009/2/20/PolaroidTeam.png]</td>
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          <td><html><a href="http://igemvalencia.questionpro.com" target="_blank"> <img src="https://static.igem.org/mediawiki/2009/f/fc/Survey_iGEM09.jpg" width="201" height="201"></a></html></td>
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'''Aequorin''' is a luminiscent protein isolated from luminescent jellyfish and many other marine organisms that uses '''coelenterazine''' as its cofactor to produce light whenever Ca<SUP>2+</SUP> is bound to it. Using a '''chemical input''' (KOH, alkali shock) we will open '''Ca<SUP>2+</SUP> channels''' in the yeasts' membrane (Viladevall L, et al. J Biol Chem. (2004) 279 43614–43624), Ca<SUP>2+</SUP> will enter into the cells and we will get '''light''' as an '''output'''.
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          <td><html><a href="https://2009.igem.org/Team:Valencia/Human" target="_top"> <img src="https://static.igem.org/mediawiki/2009/a/a5/V_Ranking.jpg" width="260" height="140" ></a></html></td>
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Later we will try to reproduce the same response with an '''electrical stimulus''', using some hardware built by ourselves.
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Latest revision as of 13:00, 21 September 2009


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