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Team:Warsaw/Calendar-Main/21 August 2009
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(New page: {{WarNotebook}} <!-- do not edit above me! --> <html> <h3><div style="text-align: center;">Assembly of endosomal detection operon</div></h3> <h4>Marcin</h4> <br/> <p>Task 1:</p><ul><li>Pre...) |
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<li>p53 CDS + <a href="http://partsregistry.org/Part:BBa_B0032"><span style="color: black">BBa_B0032</a></span> on <a href="http://partsregistry.org/Part:pSB1A3"><span style="color: black">pSB1A3</a></span> plasmid</li> | <li>p53 CDS + <a href="http://partsregistry.org/Part:BBa_B0032"><span style="color: black">BBa_B0032</a></span> on <a href="http://partsregistry.org/Part:pSB1A3"><span style="color: black">pSB1A3</a></span> plasmid</li> | ||
<li>cro CDS + <a href="http://partsregistry.org/Part:pSB1A3"><span style="color: black">pSB1A3</a></span> plasmid</li></ul></ul> | <li>cro CDS + <a href="http://partsregistry.org/Part:pSB1A3"><span style="color: black">pSB1A3</a></span> plasmid</li></ul></ul> | ||
| + | <br/> | ||
| + | <p>Digest of isolated plasmids and transformation of chemocompetent E. coli strain DH5&alpha with <a href="http://partsregistry.org/Part:BBa_C0040"><span style="color: black">BBa_C0040</a></span> + <a href="http://partsregistry.org/Part:BBa_R0080"><span style="color: black">BBa_R0080</a></span> on <a href="http://partsregistry.org/Part:pSB1A3"><span style="color: black">pSB1A3</a></span> were done by Kuba<p> | ||
| - | <p> | + | <html> |
| - | + | <h3><div style="text-align: center;">Making of the plac-RBS-llo part</div></h3> | |
| + | <h4>Jarek</h4> | ||
| + | <br /> | ||
| + | <p>Tasks:</p> | ||
| + | <ul> | ||
| + | <li>Separation of digestion products from 20 August in 0,8% agarose gel. </li> | ||
| + | <li>Digestion of 8 plasmid DNA samples with XbaI and PstI endonucleases.</li> | ||
| + | <li>Separation of digestion products in 0,8% agarose gel.</li> | ||
| + | <li>Transformation competent ''E.coli'' strain with DNA sample containing plac+RBS+llo part and plating the transformated culture on the LB+Amp plates. </li> | ||
| + | </ul> | ||
| + | <br /> | ||
| + | <h3><div style="text-align: center;">Cloning switch 1 regulatory parts [ | ||
| + | <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K177012">K177012</a>-PcI.RBS.LacI, | ||
| + | <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K177033">K177033</a>-PcI.RBS.LacI.PcI.RBS.RFP.terminator, | ||
| + | <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K177011">K177011</a>-PLacI.RBS.cI.terminator, | ||
| + | <a href="http://partsregistry.org/Part:BBa_K177038">K177038</a>-PLacI.RBS.cI.terminator.PLacI.RBS.GFP.terminator | ||
| + | ] | ||
| + | into two compatible low copy number plasmids of different antibiotic resistance</div></h3> | ||
| + | <h4>Ania</h4> | ||
| + | <br/> | ||
| + | <p>Tasks:</p> | ||
| + | <ul> | ||
| + | <li> | ||
| + | </li> | ||
| + | </ul> | ||
| + | <br/> | ||
</html> | </html> | ||
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Latest revision as of 00:12, 10 September 2009
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Assembly of endosomal detection operon
Marcin
Task 1:
- Prepare the bacterial cultures for isolation of following constructs:
Digest of isolated plasmids and transformation of chemocompetent E. coli strain DH5&alpha with BBa_C0040 + BBa_R0080 on pSB1A3 were done by Kuba
Making of the plac-RBS-llo part
Jarek
Tasks:
- Separation of digestion products from 20 August in 0,8% agarose gel.
- Digestion of 8 plasmid DNA samples with XbaI and PstI endonucleases.
- Separation of digestion products in 0,8% agarose gel.
- Transformation competent ''E.coli'' strain with DNA sample containing plac+RBS+llo part and plating the transformated culture on the LB+Amp plates.
Ania
Tasks:
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