|
|
| (65 intermediate revisions not shown) |
| Line 1: |
Line 1: |
| - | {| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center" | + | {{:Team:Washington-Software/Header|in}} |
| - | !align="center";style="border: none;" |[[Image:WashingtonColorSeal-21-clip.gif|50px]]
| + | |
| - | !align="center"; style="border: #6b0c6a inset 3px;" |[[Team:University_of_Washington|<font color="gold">Home</font>]]
| + | |
| - | !align="center"|[[Team:Washington-Software|Home]]
| + | |
| - | !align="center"|[[Team:Washington-Software/Team|The Team]]
| + | |
| - | !align="center"|[[Team:Washington-Software/Project|The Project]]
| + | |
| - | !align="center"|[[Team:Washington-Software/Parts|Temp home]]
| + | |
| - | !align="center"|[[Team:Washington-Software/Modeling|Modeling]]
| + | |
| - | !align="center"|[[Team:Washington-Software/Notebook|Notebook]]
| + | |
| - | |} | + | |
| | | | |
| - | <html> | + | <h2> Abstract </h2> |
| - | <head>
| + | |
| - | <style>
| + | |
| - | #globalWrapper {
| + | |
| - | margin: 0px;
| + | |
| - | padding: 0px;
| + | |
| - | background-color:#500050;
| + | |
| - | }
| + | |
| - | #content{
| + | |
| - | background:#FFdd00;
| + | |
| - | border-left-color:#FFdd00;
| + | |
| - | border-right-color:#FFdd00;
| + | |
| - | }
| + | |
| - | #footer-box {
| + | |
| - | border-top-color:#FFdd00;
| + | |
| - | border-right-color:#FFdd00;
| + | |
| - | border-bottom-color:#FFdd00;
| + | |
| - | border-left-color:#FFdd00;
| + | |
| - | background:#FFdd00;
| + | |
| - | }
| + | |
| - | </style>
| + | |
| - | </head>
| + | |
| - | </html> | + | |
| | | | |
| - | {| style="color:Gold;background-color:#500050;border: none" cellpadding="4" cellspacing="5" border="5" width="99%" align="center"
| + | <h4>'''BioBrick-A-Bot: Lego Robot for Automated BioBrick DNA Assembly'''</h4> |
| - | !align="center";style="border: none;" |[[Image:WashingtonColorSeal-21-clip.gif|50px]]
| + | [[Image:Robot Close Up.jpg|thumb|right|'''BioBrick-A-Bot''']] |
| - | !align="center"; style="border: #6b0c6a inset 3px;" |[[Team:University_of_Washington|<font color="gold">Home</font>]]
| + | |
| - | !align="center"; style="border: #6b0c6a outset 3px;" |[[Team:University_of_Washington/Team|<font color="gold">The Team</font>]]
| + | |
| - | !align="center"; style="border: #6b0c6a outset 3px;" |[[Team:University_of_Washington/Project|<font color="gold">The Project</font>]]
| + | |
| - | !align="center"; style="border: #6b0c6a outset 3px;" |[[Team:University_of_Washington/Modeling|<font color="gold">Modeling</font>]]
| + | |
| - | !align="center"; style="border: #6b0c6a outset 3px;" |[[Team:University_of_Washington/Notebook|<font color="gold">Notebook</font>]]
| + | |
| - | !align="center"; style="border: #6b0c6a outset 3px;" |[[Team:University_of_Washington/Protocols|<font color="gold">Protocols</font>]]
| + | |
| - | !align="center"; style="border: #6b0c6a outset 3px;" |[[Team:University_of_Washington/Parts|<font color="gold">Parts Submitted <br>to the Registry</font>]]
| + | |
| - | !align="center"; style="background-color:#03943d;border: #6b0c6a outset 3px;" |[[Team:University_of_Washington/Measurement Kit|<font color="gold">Measurement Kit</font>]]
| + | |
| - | !align="center"; style="background-color:#c42317;border: #6b0c6a outset 3px;" |[[Team:University_of_Washington/SeToB|<font color="gold">SeToB</font>]]
| + | |
| - | !align="center"; style="border: #6b0c6a outset 3px;" |[[Team:University_of_Washington/Safety|<font color="gold">Safety</font>]]
| + | |
| - | |}
| + | |
| | | | |
| - | {|style="color:Gold;background-color:#FFdd00; border: none" cellpadding="5" cellspacing="5" border="0" width="99%" align="center"
| + | Commercial Liquid Handling Systems are extremely expensive, and are typically beyond the reach of the average molecular biologist interested in performing high throughput methods. To address this problem, we design and implement a liquid handling system built from commonly accessible Legos. Our goal is the automation of BioBrick assembly on a platform that can itself be easily replicated and we demonstrate a proof-of-principle for this system by transferring colored dye solutions on a 96-well plate. We introduce a new concept called LegoRoboBrick. The liquid handling system is build from 3 new LegoRoboBrick modular components: ALPHA (Automated Lego Pipette Head Assembly), BETA (BioBrick Environmental Testing Apparatus), and PHI (Pneumatic Handling Interface). We will demonstrate that the same BioBrick assembly software can run on multiple plug-and-play LegoRoboBrick instances with different physical dimensions and geometric configurations. The modular design of LegoRoboBricks allows easy extension of new laboratory functionalities in the future. |
| - | |
| + | |
| - | <h1> Abstract </h1>
| + | |
| | | | |
| - | <h5><big>'''LegoRoboBricks for Automated BioBrick Assembly'''</big> | + | <h2> Project Goals </h2> |
| | + | #Low Cost – Robot cost significantly lower than $10,000, the price of a commercial liquid handling robot. (Actual cost of BioBrick-a-Bot prototype: ~$700) |
| | + | #Hardware Platform that is easily accessible. |
| | + | #Hardware Design that is easily replicable by other iGEM teams. |
| | + | #Software Design that is robust, plug and play. Can swap modules from other iGEM teams. |
| | + | #Design that is easily extensible, to allow future collaboration with other iGEM teams. |
| | | | |
| | + | <h2> The Vision </h2> |
| | | | |
| | + | [[Image:LegoRoboBrick.jpg|315px|left]][[Image:LegoRoboBrick2.jpg|315px|left]][[Image:LegoRoboBricks.jpg|313px|right]] |
| | | | |
| - | Commercial Liquid Handling Systems are extremely expensive, and are typically beyond the reach of the average molecular biologist interested in performing high throughput methods. To address this problem, our project consists of the design and implementation of a liquid handling system built from commonly accessible Legos. We demonstrate a proof-of-principle use for this system to perform BioBrick assembly by transferring colored dye solutions on a 96-well plate.
| + | <h2> Acknowledgements </h2> |
| | | | |
| - | We introduce a new concept called LegoRoboBrick. The liquid handling system is build by designing and implementing 3 LegoRoboBrick modular components: ALPHA (Automated Lego Pipette Head Assembly), BETA (BioBrick Environmental Testing Apparatus), and PHI (Pneumatic Handling Interface). We will demonstrate that the same BioBrick assembly software can run on multiple plug-and-play LegoRoboBrick instances with different physical dimensions and geometric configurations. The modular design of LegoRoboBricks allows easy extension of new laboratory functionalities in the future. </h5>
| + | Our iGEM project is sponsored by the [http://depts.washington.edu/bioe/ BioEngineering Department] at the [http://www.washington.edu/ University of Washington] |
| - | |[[Image:legorobobricks.jpg|400px]]
| + | |
| - | |}
| + | |
| | | | |
| - | ==Check list==
| + | [[Image:bioelogo.jpg]] |
| - | Home: the whole picture of the robot, abstract, project goals
| + | |
| - | Team: photos of everyone, group picture
| + | |
| - | project: photos of the robot from different angles,video source from you tube
| + | |
| - | diagrams (powerpoint etc), more explanation on each module (story...background...)
| + | |
| - | Modeling:
| + | |
| - | Notebook: notes that has been taken, including the codes
| + | |
| - | Timeline(?)
| + | |
| | | | |
| - | | + | [[Image:uw_logo.jpg]] |
| - | | + | |
| - | | + | |
| - | <!-- *** What falls between these lines is the Alert Box! You can remove it from your pages once you have read and understood the alert *** -->
| + | |
| - | | + | |
| - | <html>
| + | |
| - | <div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
| + | |
| - | <div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
| + | |
| - | This is a template page. READ THESE INSTRUCTIONS.
| + | |
| - | </div>
| + | |
| - | <div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
| + | |
| - | You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
| + | |
| - | </div>
| + | |
| - | <div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
| + | |
| - | You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, and a lab notebook. PLEASE keep all of your pages within your teams namespace.
| + | |
| - | </div>
| + | |
| - | </div>
| + | |
| - | </html>
| + | |
| - | | + | |
| - | <!-- *** End of the alert box *** -->
| + | |
| - | [[Image:Example.jpg]] | + | |
| - | | + | |
| - | | + | |
| - | <!--- The Mission, Experiments --->
| + | |
Commercial Liquid Handling Systems are extremely expensive, and are typically beyond the reach of the average molecular biologist interested in performing high throughput methods. To address this problem, we design and implement a liquid handling system built from commonly accessible Legos. Our goal is the automation of BioBrick assembly on a platform that can itself be easily replicated and we demonstrate a proof-of-principle for this system by transferring colored dye solutions on a 96-well plate. We introduce a new concept called LegoRoboBrick. The liquid handling system is build from 3 new LegoRoboBrick modular components: ALPHA (Automated Lego Pipette Head Assembly), BETA (BioBrick Environmental Testing Apparatus), and PHI (Pneumatic Handling Interface). We will demonstrate that the same BioBrick assembly software can run on multiple plug-and-play LegoRoboBrick instances with different physical dimensions and geometric configurations. The modular design of LegoRoboBricks allows easy extension of new laboratory functionalities in the future.
"
