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Uppsala-Sweden/11 October 2009
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(New page: ==Plasmid prep of PRPRAT and PRKRYT== data shown here:Media:091011_Plasmidprep.xls ==Triparentalmating== Today we put our antisense-RNA constructs in to Synecocystis via triparental m...) |
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==Plasmid prep of PRPRAT and PRKRYT== | ==Plasmid prep of PRPRAT and PRKRYT== | ||
| - | |||
| - | == | + | data shown here: [[Media:091011_Plasmidprep.xls]] |
| + | |||
| + | ==Triparental mating== | ||
| + | |||
Today we put our antisense-RNA constructs in to Synecocystis via triparental mating. | Today we put our antisense-RNA constructs in to Synecocystis via triparental mating. | ||
| - | + | Protocol here: [[Media:Triparental+Mating.txt]] | |
| + | |||
| + | Deviated in that we did not concentrate the Synecocystis culture and instead just it straight and at 10 times dilution, corresponding to 10 and 100 times dilution in the protocol. | ||
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| + | ==Evaluating constructs in ''e.coli''== | ||
| + | |||
| + | The transformations made yesterday gave plenty of colonies and some were picked and verified in a colony PCR. | ||
| + | |||
| + | {{Uppsala-Sweden_Footer}} | ||
Latest revision as of 16:11, 21 October 2009
Plasmid prep of PRPRAT and PRKRYT
data shown here: Media:091011_Plasmidprep.xls
Triparental mating
Today we put our antisense-RNA constructs in to Synecocystis via triparental mating. Protocol here: Media:Triparental+Mating.txt
Deviated in that we did not concentrate the Synecocystis culture and instead just it straight and at 10 times dilution, corresponding to 10 and 100 times dilution in the protocol.
Evaluating constructs in e.coli
The transformations made yesterday gave plenty of colonies and some were picked and verified in a colony PCR.
