From 2009.igem.org
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| | -[[User:Bussingkm|Bussingkm]] 00:21, 7 August 2009 (UTC) | | -[[User:Bussingkm|Bussingkm]] 00:21, 7 August 2009 (UTC) |
| | | | |
| | + | ''Maria and Afton'' |
| | + | * Overnight culture was unsuccessful because not all of the parts were prepared for an overnight culture. Only pSB1C3 was picked from a plate. The other parts, J06702 and a promoter were not grown up so all parts will be grown up today. |
| | + | [[User:Trentay|Trentay]] 01:12, 7 August 2009 (UTC) |
| | ---- | | ---- |
| | ===Tasks=== | | ===Tasks=== |
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| | *I need to finish writing a progress report for my fellowship. Hopefully the flow cytometer will be fixed soon so that we can finally get some results. Confirmed that whenever we have florescing bacteria ready we can proceed with RT PCR. | | *I need to finish writing a progress report for my fellowship. Hopefully the flow cytometer will be fixed soon so that we can finally get some results. Confirmed that whenever we have florescing bacteria ready we can proceed with RT PCR. |
| | -[[User:Bussingkm|Bussingkm]] 00:21, 7 August 2009 (UTC) | | -[[User:Bussingkm|Bussingkm]] 00:21, 7 August 2009 (UTC) |
| | + | |
| | + | ''Maria and Afton'' |
| | + | * Make overnight culture of J06702 and J23100/110 from frozen stocks |
| | + | * Pick colony from pSB1C3 for overnight culture |
| | + | [[User:Trentay|Trentay]] 01:12, 7 August 2009 (UTC) |
| | ---- | | ---- |
| | ====Wetlab==== | | ====Wetlab==== |
| | * Picked colonies for several new plasmids that came in today. The plasmids contained parts: | | * Picked colonies for several new plasmids that came in today. The plasmids contained parts: |
| - | **BBa_I313600 | + | **BBa_I13600 tetR promoter with CFP RET |
| - | **BBa_K118024 | + | **BBa_K118024 Craig and Clay's pathway sequence |
| | **BBa_K118025 | | **BBa_K118025 |
| | **BBa_I742111 | | **BBa_I742111 |
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| | | | |
| | -[[User:Bussingkm|Bussingkm]] 00:21, 7 August 2009 (UTC) | | -[[User:Bussingkm|Bussingkm]] 00:21, 7 August 2009 (UTC) |
| | + | |
| | + | ''Maria and Afton'' |
| | + | * Overnight cultures were made |
| | + | [[User:Trentay|Trentay]] 01:12, 7 August 2009 (UTC) |
Latest revision as of 01:12, 7 August 2009
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Thursday 6 August 2009
Results
Kevin
- Everything that I digested, ligated, and transformed yesterday grew over night. However, I used the wrong plasmid backbone. Both my reporter and backbone insert reported RFP. Will be growing same backbone containing the death gene (BBa_P1010). We will use E. coli strain Db3.1 to grow plasmid. The DNA will be minipreped Friday and digested and ligated on Monday.
-Bussingkm 00:21, 7 August 2009 (UTC)
Maria and Afton
- Overnight culture was unsuccessful because not all of the parts were prepared for an overnight culture. Only pSB1C3 was picked from a plate. The other parts, J06702 and a promoter were not grown up so all parts will be grown up today.
Trentay 01:12, 7 August 2009 (UTC)
Tasks
Kevin
- I need to finish writing a progress report for my fellowship. Hopefully the flow cytometer will be fixed soon so that we can finally get some results. Confirmed that whenever we have florescing bacteria ready we can proceed with RT PCR.
-Bussingkm 00:21, 7 August 2009 (UTC)
Maria and Afton
- Make overnight culture of J06702 and J23100/110 from frozen stocks
- Pick colony from pSB1C3 for overnight culture
Trentay 01:12, 7 August 2009 (UTC)
Wetlab
- Picked colonies for several new plasmids that came in today. The plasmids contained parts:
- BBa_I13600 tetR promoter with CFP RET
- BBa_K118024 Craig and Clay's pathway sequence
- BBa_K118025
- BBa_I742111
All parts came on the high copy, ampicillin resistant backbone pSB1A2
-Bussingkm 00:21, 7 August 2009 (UTC)
Maria and Afton
- Overnight cultures were made
Trentay 01:12, 7 August 2009 (UTC)
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