August/14 August 2009

From 2009.igem.org

miniprep

Conducted a "miniprep" to harvest and check concentration of plasmids from 14 sample.Result of Nano drop concentration check :(sample No.) (concentration) [260/280] [260/230]

  R0071 (1-12C)   29.5ng/μL  1.56  0.91
  R0040 (1-6I)     7.5        1.88  2.79
  R0079 (1-12A)   11.5       1.92  2.65
  I14017 (1-18L)   28.6       1.81  2.46
  B0034 (1-2M)    10.1       1.96  2.60
  R0078 (1-16G)   14.0       1.99  2.47
  C0171 (2-8I)     15.4       1.86  3.29
  F2621 (2-21F)   17.9       1.99  2.60
  I14015 (2-11N)   11.2       1.88  2.32
  F1610 (2-24G)   19.5       1.97  2.34
  C0161 (2-8O)    11.0       1.60  1.74
  F2620 (2-6E)     5.5        1.46  2.03
  K204001 - 3    16.1       1.78  2.40
  K204001 - 8    14.4       1.77  2.38

transformation

to get new plasmid

   sample No.
    ③ ④ ⑤ ⑥

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