August/14 August 2009
From 2009.igem.org
miniprep
Conducted a "miniprep" to harvest and check concentration of plasmids from 14 sample.Result of Nano drop concentration check :(sample No.) (concentration) [260/280] [260/230]
R0071 (1-12C) 29.5ng/μL 1.56 0.91 R0040 (1-6I) 7.5 1.88 2.79 R0079 (1-12A) 11.5 1.92 2.65 I14017 (1-18L) 28.6 1.81 2.46 B0034 (1-2M) 10.1 1.96 2.60 R0078 (1-16G) 14.0 1.99 2.47 C0171 (2-8I) 15.4 1.86 3.29 F2621 (2-21F) 17.9 1.99 2.60 I14015 (2-11N) 11.2 1.88 2.32 F1610 (2-24G) 19.5 1.97 2.34 C0161 (2-8O) 11.0 1.60 1.74 F2620 (2-6E) 5.5 1.46 2.03 K204001 - 3 16.1 1.78 2.40 K204001 - 8 14.4 1.77 2.38
transformation
to get new plasmid
sample No. ③ ④ ⑤ ⑥