August/16 October 2009

From 2009.igem.org

1. min prep

sample    conc.
  97            98 ng/uL
  97            82
  98            66
  98            56
 101           57
 102           33
  87            30

2. digation

K204085

Vector		Insert
40	3	1-15L	10
EcoRI	1	EcoRI	1
XbaI	1	SpeI	1
No.2	5	No.2	5
dH₂O	35	dH₂O	28
total	50uL	total	50uL

K204087

Vector		Insert
62	2	47	6
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH₂O	36	dH₂O	32
total	50uL	total	50uL

K204088

Vector		Insert
X3	2	69	3
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH₂O	36	dH₂O	35
total	50uL	total	50uL

K2040103

Vector		Insert
95	7	65	1
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH₂O	8	dH₂O	35
total	20uL	total	20uL

↓
37 degree , 2hr
gel cut&purification
ligation

3.min prep

luxR - FliH    167 ng/uL
                    215 ng/uL

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