"
August/1 October 2009
From 2009.igem.org
1.colony check
sample no. no. of colony
41 +++
42 +++
48 +++
1-1K +++
1-6I +++
57 +
2.min prep
sample no. conc.
52 61.4 ng/uL
3.fluorence check (color parts)
not confirm fluorence...
4.prepared LA plate
5.transformation
sample 1-14N , 1-16I
6.digation
K204058
| Vector | Insert | ||
|---|---|---|---|
| 1-8E | 4 | 1-8I | 10 |
| SpeI | 1 | XbaI | 1 |
| PstI | 1 | PstI | 1 |
| No.2 | 2 | No.2 | 2 |
| dH2O | 12 | dH2O | 6 |
| total | 20uL | total | 20uL |
K204059
| Vector | Insert | ||
|---|---|---|---|
| 1-8E | 9 | 1-8K | 2 |
| SpeI | 1 | XbaI | 1 |
| PstI | 1 | PstI | 1 |
| No.2 | 2 | No.2 | 2 |
| dH2O | 7 | dH2O | 14 |
| total | 20uL | total | 20uL |
K204060
| Vector | Insert | ||
|---|---|---|---|
| 1-8E | 8 | 1-12M | 4 |
| SpeI | 1 | XbaI | 1 |
| PstI | 1 | PstI | 1 |
| No.2 | 2 | No.2 | 2 |
| dH2O | 8 | dH2O | 12 |
| total | 20uL | total | 20uL |
K204061
| Vector | Insert | ||
|---|---|---|---|
| 1-8E | 8 | 2-8E | 8 |
| SpeI | 1 | XbaI | 1 |
| PstI | 1 | PstI | 1 |
| No.2 | 2 | No.2 | 2 |
| dH2O | 8 | dH2O | 12 |
| total | 20uL | total | 20uL |
K204062
| Vector | Insert | ||
|---|---|---|---|
| 1-8E | 9 | 33 | 1 |
| SpeI | 1 | XbaI | 1 |
| PstI | 1 | PstI | 1 |
| No.2 | 2 | No.2 | 2 |
| dH2O | 7 | dH2O | 15 |
| total | 20uL | total | 20uL |
K204063
| Vector | Insert | ||
|---|---|---|---|
| 1-23L | 6 | 1-4E | 9 |
| EcoRI | 1 | EcoRI | 1 |
| XbaI | 1 | SpeI | 1 |
| No.2 | 2 | No.2 | 2 |
| dH2O | 10 | dH2O | 7 |
| total | 20uL | total | 20uL |
↓
37°C, O/N
7.min prep
sample conc. X1 261.1 ng/uL X2 301.5 X3 345.9 X4 360 X5 192 X6 219 X7 319 X8 353 X4 334
