"
August/2 October 2009
From 2009.igem.org
1.colony check
sample no. of colony 1-14N 0 1-16I 0
2.digation
K204064
| Vector | Insert | ||
|---|---|---|---|
| 2-16N | 3 | 2-18H | 6 |
| EcoRI | 1 | EcoRI | 1 |
| XbaI | 1 | SpeI | 1 |
| No.2 | 2 | No.2 | 2 |
| dH2O | 13 | dH2O | 10 |
| total | 20uL | total | 20uL |
K204065
| Vector | Insert | ||
|---|---|---|---|
| 2-16L | 2 | 2-18F | 10 |
| SpeI | 1 | XbaI | 1 |
| PstI | 1 | PstI | 1 |
| No.2 | 2 | No.2 | 2 |
| dH2O | 14 | dH2O | 6 |
| total | 20uL | total | 20uL |
K204066
| Vector | Insert | ||
|---|---|---|---|
| 1-19B | 2 | 2-18F | 10 |
| EcoRI | 1 | EcoRI | 1 |
| XbaI | 1 | SpeI | 1 |
| No.2 | 2 | No.2 | 2 |
| dH2O | 14 | dH2O | 6 |
| total | 20uL | total | 20uL |
↓
37 drgree O/N
3.ligation
sample no. 63 , 64 , 65
4.transformation
sample no. 1-14N , 1-16I , 2-18F
