Duke University/16 June 2009

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June 16, 2009

  • E: PCR with Bioplastics #2 as template and Phusion mix (done by Maggie), Gels to check PCRs
2x Phusion mix 25 ul
forward primer 2.5 ul
reverse primer 2.5 ul
DNA template (plasmid) 1 ul
H2O 19 ul
50 ul
  • R: No bands for fragments 3, Bands for fragment 4
  • C: Cut out bands of fragment 4, PCR again to obtain fragment 3


  • E: Measured concentration of DNA fragments 1 and 2
  • R: Concentrations okay
  • C: Do assembly PCR once enough of fragments 3 and 4 obtained