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HKU-HKBU/5 August 2009
From 2009.igem.org
Contents |
Co-transformation
12:50 pre-culture MG3 in rotator (32℃) for 3h
15:50 take bacteria out from rotator
16:10 sub-culture MG3 in warm room (37℃) for 1.5h
17:40 take bacteria out from warm room
Competent Cell Preparation Electro-transformation ① A+ & C+ ② A+ & C+ ③ A+ ④ C+ After transformation, recovering (1h) and spreading plates, put these plates into warm room (37℃) for overnight Suppose: ① A+ & C+ →grow ② A+ & C+ →grow ③ A+ →grow ④ C+ →grow
