Minnesota-experimental/15 July 2009

From 2009.igem.org

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- Flow cytometry data was collected for samples from 7-13. These included DH5αPro cells containing TNN and TTN promoter constructs mutant at position 4, as well as TOP10 cells with the same constructs as a control.

- Samples were collected from DH5αPro cells containing the TNN promoter constructs mutant at position 5. Aliquots of culture were collected and fixed 3, 6, 9, and 12 hours following innoculation of the cultures in media containing various concentrations of aTc and IPTG as inducers. TOP10 cells containing the same constructs were also sampled as a control.