Minnesota-experimental/26 June 2009

From 2009.igem.org

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Plasmids were isolated from cultures of TOP10 cells containing TNN, TTN, and TTL promoter constructs mutant at position 4. Reaction mixtures containing plasmid and a primer complimentary to the plasmid were prepared and submitted to the Biomedical Genomic Center at the University of Minnesota for DNA sequencing.