"
Minnesota-experimental/6 July 2009
From 2009.igem.org
| Back to Notebook Home | |
| Go to Previous Day (July 5) | Go to Next Day (July 7) |
- TNN, TTN, and TTL promoter constructs mutant at position 6 were constructed by SOEing PCR.
- SOEing PCR products were cleaned using a QIAquick column protocol. Cleaned products were amplified by PCR and stored at 4°C.
- Plasmids containing TNN, TTN promoter constructs mutant at position 4 were transformed into DH5αPro cells. Transformed cells were spread on antibiotic LB agar plates and grown overnight at 37 °C.
