Minnesota/21 July 2009

From 2009.igem.org

Back to Notebook Home
Go to Previous Day (July 20)Go to Next Day (July 22)

smad_ben did not run properly. The issue that Ben was having was eliminated, so I never reran the model.

Today I looked at how the tetR2 concentrations are behaving as a possible reason why I am finding it difficult to shift the point at which GFP concentration reaches a steady state. The issue I think is that tetR2 splits on division, and thus is diluted if tetR2 exists in appreciable quantities in the cell. This occurs at low aTc concentrations (as noted previously in the calendar). But tetR2:aTc and tetR2:aTc2 do not split on division. This is to avoid eliminating aTc from the system. So at high aTc concentrations tetR2 is much more prevalent. This problem will be explored further in the coming days.