From 2009.igem.org
Today the first model of the TNN system was created. In order to do this, the previous work of the Kaznessis group was used to provide a basis for a a lot of the reactions. This allowed us to easily obtain reasonable kinetic constants for almost all the reactions in the model.
Here is the first incarnation of the model (many future models will simply change this one):
| Reaction
| Forward Kinetic Constant
| Reverse Kinetic Constant
|
| RNAp + lacP + tetR2:tetO1 -> RNAp:lacP | 6.23E+05 |
|
| RNAp + lacP + tetO1 ↔ RNAp:lacP | 1E+07 | 1
|
| RNA:lacP -> RNAp:lacP* | .01 |
|
| RNAp:lacP* -> lacP + tetO1 + RNAp:DNAgfp | 30 |
|
| RNAp:DNAgfp -> RNAp + gfp_mRNA | 30 |
|
| gfp_mRNA + rib -> rib:gfp_mRNA | 100000 |
|
| rib:gfp_mRNA -> rib:gfp_mRNA_1 + gfp_mRNA | 33 |
|
| rib:gfp_mRNA_1 -> rib + gfp | 33 |
|
| tetR2 + aTc ↔ tetR2:aTc | 2E+09 | 4E-04
|
| tetR2:aTc + aTc ↔ tetR2:aTc2 | 1E+08 | 1E-03
|
| tetR2:aTc + tetO1 ↔ tetR2:tetO1:aTc | 1E+08 | 1
|
| tetR2:aTc2 + tetO1 ↔ tetR2:tetO1:aTc2 | 1E+08 | 1E+05
|
| tetR2:tetO1 + aTc ↔ tetR2:tetO1:aTc | 1E+08 | 1E-03
|
| tetR2:tetO1:aTc + aTc ↔ tetR2:tetO1:aTc2 | 1E+08 | 1E-03
|
| tetR2 -> Ø | 2.89E-04 |
|
| tetR2:aTc -> aTc | 2.89E-04 |
|
| tetR2:aTc2 -> 2 aTc | 2.89E-04 |
|
| tetR2 + nsDNA ↔ tetR2:nsDNA | 1000 | 3.2409
|
| tetR2:aTc + nsDNA ↔ tetR2:aTc:nsDNA | 1000 | 3.2409
|
| tetR2:aTc:nsDNA -> aTc + nsDNA | 1.93E-04 |
|
| tetR2:nsDNA -> nsDNA | 1.93E-04 |
|
| Ø -> tetR2 | 1E-11 |
|
| Ø -> aTc | 3.3E-04 |
|
| gfp_mRNA -> Ø | 1.16E-03 |
|
| gfp -> Ø | 3.21E-05 |
|
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