"
September/19 September 2009
From 2009.igem.org
1.min prep
result of nano drop
sample no. conc.
29A 18.2 ng/uL
29B 25.7
19A 19.2
19B 15.7
2.cut check & ligation
gel electrophoresis
19 : ligation OK
3.transformation
sample no. 27 , 28 , 30 , 35 , 48 , 50 , 51
45 , 46
1-1F
4.digation
K204036
| Vector | Insert | ||
|---|---|---|---|
| 3-1M | 5 | 1-12M | 5 |
| SpeI | 1 | XbaI | 1 |
| PstI | 1 | PstI | 1 |
| No.2 | 2 | No.2 | 2 |
| dH2O | 11 | dH2O | 11 |
| total | 20uL | total | 20uL |
K204037
| Vector | Insert | ||
|---|---|---|---|
| 3-1M | 5 | 1-8K | 5 |
| SpeI | 1 | XbaI | 1 |
| PstI | 1 | PstI | 1 |
| No.2 | 2 | No.2 | 2 |
| dH2O | 11 | dH2O | 11 |
| total | 20uL | total | 20uL |
K204038
| Vector | Insert | ||
|---|---|---|---|
| 3-1M | 5 | 1-8I | 11 |
| SpeI | 1 | XbaI | 1 |
| PstI | 1 | PstI | 1 |
| No.2 | 2 | No.2 | 2 |
| dH2O | 11 | dH2O | 5 |
| total | 20uL | total | 20uL |
K204039
| Vector | Insert | ||
|---|---|---|---|
| 3-1M | 4 | 2-8E | 5 |
| SpeI | 1 | XbaI | 1 |
| PstI | 1 | PstI | 1 |
| No.2 | 2 | No.2 | 2 |
| dH2O | 12 | dH2O | 11 |
| total | 20uL | total | 20uL |
↓
37°C, O/N
