September/22 September 2009

From 2009.igem.org

1. colony check

 sample no   no. of colony
   36              5
   37              0
   38              1
   29              0

2.min prep

sample no       conc.
  3-7G          94.8 ng/uL
  2-3I          114
  1-8K          144
  2-18F         120
                136
  46            77
  2-9A          132
  3-7C          149
  27B           104
  29A           7.9
  46            65

3.transfromation

sample no.
X1-A , X2-A , X3-A
1-12M

4.digestion

K204028

Vector		Insert
2-18H	3	2-16N	8
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH₂O	13	dH₂O	8
total	20uL	total	20uL

K204030

Vector		Insert
2-18F	2	1-19B	5
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH₂O	14	dH₂O	11
total	20uL	total	20uL

↓
37°C, 2hr
↓
gel electrophoresis
gel cut
purification
↓
ligation O/N

5.transformation

sample no.
  22 , 25 , 40 , 47

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