September/29 September 2009

From 2009.igem.org

1.colony check

sample no    no. of colony
  36              5
  37              5
  38              3
  39              1
  53              1

2.digation

K204057

Vector		Insert
1-23L	7	35	2
EcoRI	1	EcoRI	1
XbaI	1	SpeI	1
No.2	2	No.2	2
dH₂O	9	dH₂O	14
total	20uL	total	20uL

↓
37degree , 3hr
Agarose Gel Electro-phoresis
gel cut
purification
ligation

3.transformation

sample No. 52

4.colony check

sample no    no. of colony
  28              1
  29              1
  20              3

  11              3
  X1              4
  X2              6
  X3             10
  X4              1
  X4              6
  X5             <30
  X6             10
  X7              2
  X8              3

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