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Team:Newcastle/Cadmium sensing
==Cadmium Sensing== ...ranscription to occur. These proteins therefore allow selective sensing of cadmium in the form of an AND gate, a model for which can be seen in our modelling

711 B (106 words) - 09:39, 22 July 2009
Team:Newcastle/Cadmium
=Why cadmium?= The BacMan project is focused mainly on sequestering the heavy metal cadmium from contaminated soils, however with the many other heavy metals known to

4 KB (684 words) - 12:59, 31 July 2009

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Team:Newcastle/Project
...rporated into a ''Bacillus'' spore, the resilience of which means that the cadmium ions can become isolated from the environment (and made bio-unavailable) fo # [https://2009.igem.org/Team:Newcastle/Project#Cadmium_Intake Cadmium Intake]

16 KB (2,501 words) - 02:05, 22 October 2009
Team:Newcastle/Choices
==Why deal with cadmium?== The BacMan project is focused mainly on sequestering the heavy metal cadmium from contaminated soils, however with the many other heavy metals known to

6 KB (890 words) - 20:26, 21 October 2009
Team:Newcastle/Metalsensing
This sub-project focusses on getting the ''Bacillus subtilis'' to sense the cadmium and being able to respond to this detection. Please click on the links belo ... sensors ensures the ''Bacillus subtilis'' cells have a tightly controlled cadmium sensor, as naturally occurring metal sensitive promoters can respond to OTH

10 KB (1,512 words) - 02:54, 22 October 2009
Team:Cornell/Project
==Cadmium Contamination== ...ubstances and Disease Registry). For instance, in late 20th century Japan, cadmium contamination of water in the Jingzu River led to significant kidney proble

8 KB (1,172 words) - 03:50, 22 October 2009
Team:Newcastle/Cadmium
=Why cadmium?= The BacMan project is focused mainly on sequestering the heavy metal cadmium from contaminated soils, however with the many other heavy metals known to

4 KB (684 words) - 12:59, 31 July 2009
Team:Cornell/Project/Background
==Cadmium Contamination== ...age:Itai_itai.png|right|frame|Patient suffering from prolonged exposure to cadmium]]

5 KB (722 words) - 03:53, 22 October 2009
Jamboree/Project Abstract/Team Abstracts
...gineering the Bacillus Subtilis Metal Ion Homeostasis System to Serve as a Cadmium Responsive Biosensor=== ... bacterial biosensors and are unable to measure the amount of bioavailable cadmium.

105 KB (15,465 words) - 15:13, 5 November 2009
Team:Newcastle
'''Cadmium contamination''' can be a serious problem in countries where polluting indu ...ble'''. Germination of the spores can be disabled, making retrieval of the cadmium unnecessary since they can persist intact for thousands of years.

9 KB (1,304 words) - 11:37, 9 November 2009
Team:Newcastle/Modeling/Population/Pseudo/Bacteria Code
* importCadmium - import cadmium from the environment (MntH) * exportCadmium - export cadmium to the environment (CadA)

4 KB (704 words) - 02:44, 22 October 2009
Team:Newcastle/Stochasticity
** Decreased cadmium efflux ** Upregulation of cadmium import

11 KB (1,565 words) - 02:23, 22 October 2009
Team:Newcastle/Chassis
...o the spores of our engineered ''B. subtilis'', but what happens after the cadmium has been sequestered? ...to retrieve the sequestered cadmium? Or do we simply leave the sequestered cadmium in the spores of our engineered ''B. subtilis''?

7 KB (998 words) - 22:15, 21 October 2009
Team:Newcastle/Labwork/16 September 2009
...scovered that the attempts to transform ''E. coli'' with the ''arsR-cadA'' cadmium sensor failed, so on that same day we carried out a second attempt at trans '''The plate containing the cadmium-sensor ''E. coli'' transformants will also be studied - if there are coloni

10 KB (1,524 words) - 12:17, 20 August 2010
Team:Newcastle/Chassis/Introduction
...o the spores of our engineered ''B. subtilis'', but what happens after the cadmium has been sequestered? ...o retrieve the sequestered cadmium? Or, do we simply leave the sequestered cadmium in the spores of our engineered ''B. subtilis''?

4 KB (703 words) - 22:16, 21 October 2009
Team:Cornell/Project/Design
...nds and represses the cadA regulatory region and is released when bound by cadmium ions. [7] ==Cadmium Sensor Module 1==

4 KB (523 words) - 03:55, 22 October 2009
Team:Newcastle/Meetings/2 July 2009
** It was discussed that we first of all need to model cadmium only and THEN we can consider other metals - start from the basics. ** Need to find out how much cadmium binds to the promoter/protein per second

6 KB (957 words) - 11:50, 9 July 2009
Team:Newcastle/Safety
...e did not get to this point. The UK Government's guidance for working with cadmium are at: [http://www.hse.gov.uk/pubns/indg391.pdf HSE's guidance]. ...hrough the human alimentary canal. However, there may be a small risk from cadmium bound to the surface of the spore, which may leach from metallathionin-''co

5 KB (744 words) - 01:02, 22 October 2009
Team:KU Seoul/Notebook
...vy metals which are harmful for environment, such as arsenic, mercury, and cadmium. The purpose of this project is to detect the concentration of heavy metals It's a cadmium specific protein.

33 KB (5,505 words) - 17:15, 21 October 2009
Team:Newcastle/Metals
...e coat protein - this means that, should the stochastic switch decide, any cadmium mopped up could be sequestered into spores thereby making it unavailable. P In order to take up and keep the cadmium from the soil efficiently, we needed to think about a way to cross-link the

10 KB (1,556 words) - 03:56, 22 October 2009
Team:Cornell/Project/Chassis
...ncentration of heavy metals we grew B subtilis in various concentration of Cadmium and compared growth curves. ...tein that Cadmium influxes through increases B subtilis ability to grow in Cadmium.

2 KB (340 words) - 03:59, 22 October 2009
Team:Newcastle/Meetings/29 June 2009
*** It was discussed that we first of all need to model cadmium only and THEN we can consider other metals - start from the basics. *** Need to find out how much cadmium binds to the promoter/protein per second

4 KB (703 words) - 11:48, 9 July 2009