Annealing Anchor and Terminator Bytes

Instructions on how to anneal the complementary strands to obtain the same concentrations we used in our experiments (10μM).

What you will need:

• Anchor oligos (complementary)
• Terminator oligos (complementary)

Procedure:

To make a 10 μM Anchor solution with an A(B) overhang one must anneal Anchor_A(Anchor_B) and Anchor_Comp. To make a 10 μM Terminator solution with an A(B) overhang one must anneal Term_A(Term_B) and Term_Comp.

• Combine 10 μL of Anchor/Term_(A or B) and Anchor/Term_Comp 100 μM stock solutions into an eppendorf tube.
• Add 80 μL water or TE, mix.
• Heat solution to 95C for 2 minutes.
• Let cool to room temperature slowly.