Glycerol Stock

What you will need:

• Overnight with bacterial growth
• Screw cap tubes
• Glycerol

Procedure:

• Pipet 150 uL 50% glycerol into 3* properly labeled 1.5 ml screw cap tubes.
• Add 350 uL of overnight culture to each tube.
• Pipet up and down to gently mix.
• Flash freeze in liquid N2 or dry ice/methanol bath
• Place in -80 C freezer when frozen.

Notes

• If we make extra,then we have them if we need to send them out or want to store in several locations.