"
Team:Alberta/References/Publications/Scarless engineering of the Escherichia coli genome
From 2009.igem.org
Tamas Feher, Ildiko Karcagi, Zsuzsa Gyorfy, Kinga Umenhoffer, Balint Csorgo and Gyorgy Posfai
Methods Mol Biol. 2008;416:251-9
Abstract: E. coli K-12, being one of the best understood and thoroughly analyzed organisms, is the workhorse
of genetic, biochemical, and systems biology research, as well as the platform of choice for numerous
biotechnological applications. Genome minimization/remodeling is now a feasible approach to further
enhance its benefi cial characteristics for practical applications. Two genome engineering techniques, a
lambda Red–mediated deletion method and a suicide (conditionally replicative) plasmid-based allele
replacement procedure are presented here. These techniques utilize homologous recombination, and allow
the rapid introduction of virtually any modifi cations in the genome.
Link: NCBI
