Team:ArtScienceBangalore/Notebook/Week Three

From 2009.igem.org

Week Three

Contents

May 30th

We extracted DNA from human saliva and banana in our semi-functional, backyard science lab!

It was sort of like a pre-introduction to science which we did ourselves!


1) Extracting DNA from Banana: <<[http://hackteria.org/wiki/index.php/Read_here Read here]

2) Exracting DNA from Saliva:

Images of our DNA we extracted from saliva:

May 31st

Some interesting articles:

The Promise and Perils of Synthetic Biology:

http://www.thenewatlantis.com/publications/the-promise-and-perils-of-synthetic-biology

Molecular Synthetic Biology:

http://www.nature.com/msb/journal/v2/n1/full/msb4100104.html

The photosensitive stripes are made of bacteria:

http://www.edgarlissel.de/data/mnemosyne_II_01e.html

June 3rd

The Registry of Parts Exercise

To research a feasible idea among the various ideas that came up during the brainstorming session and present a dummy procedure on how to achieve the result on the basis of the scientific knowledge acquired in the two and a half weeks into iGEM.

Sanya: Bacteria that indicates rise in and regulates body temperature[http://hackteria.org/wiki/images/0/07/Light.jpg here]

[http://hackteria.org/wiki/index.php/Upasana Upasana]: Temperature regulating bacteria

All That Glitters: Neha's Bacterial Jewelery

June 5th

Beginning laboratory procedures: Introducing ourselves to understand working in a professional laboratory.

1) [http://hackteria.org/wiki/index.php/Bacterial_Transformation_:_The_Process Bacterial Transformation : The Process] | [http://hackteria.org/wiki/index.php/The_Bacterial_Transformation_Gallery The Bacterial Transformation Gallery]

2) Preparing the Plasmid (miniprep)

3) Digesting the DNA

4) Running the gel (Gel Electrophoresis)

5) Elution