Team:Chiba/Notebook/Calendar/7 September 2009
From 2009.igem.org
(6_September_2009 <|>8_September_2009)
Measurement of Receiver's Responce by AHL concentration (1)
- Making AHL water solution
We made 1 μM, 10 μM, 1 mM AHL water solution from 100 mM AHL water solution.
- Making plates
Using each concentration of AHL water solutions which made ealier, we made two plates per the concentration.
- prior culture
We did prior culture using glycerol stock(BW25113, ΔTdk,pCIA3-LuxR,pLux-GFP) .