Team:IPN-UNAM-Mexico/Notebook/August 7 2009

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August 7th, 2009

Digestion Digestion using EcoRI :

• plasmidicDNA..........3μl
• Buffer 2....................1μl     x30
• BSA..........................0.5μl  x30
• EcoRI enzyme......... 0.5μl   x30
• H2O..........................5μl

An electrophoresis gel was ran with the following quantities:

• 3μl marker 1kb
• Buffer 2 ................ 1μl.
• 3μl DNA+ 3μl of colorant

All samples were digested with EcoRI in order to linearized them.

Line 1 1kb | 3.1 ....* 3.4|9.1....* 9.9|9.8.. 1|1kb|*18.2....18.4|19.1*....19.4|1kb

line  2 1kb|22.1.......*22.4|24.2”   16.2” 14.1 9.4|1kb

the ones marked with * are plasmids extracted today.