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Team:KULeuven/17 September 2009
From 2009.igem.org
Project progress
Weekly meeting, presentation can be found here.
Progress of parts
[edit] Blue Light Receptor
- electroporation of logA (ligA in pBR322, a low copy plasmid).
- a PCR on pSB3K3 with primers ......
- restriction digest of pSB3K3, pBR322 and ligA with EcoRI and PstI followed by a gel electrophoresis and extraction.
- ligations of ligA (blp+GFP) with pBR322 (logA) and pSB3K3 (likA) were put overnight
- was taken from the distribution plates and plated on LB
- liquid cultures of ligY (blp) were made in the morning, grew and were miniprepped in the evening followed by a restriction digest with EcoRI and PstI
[edit] Vanillin Production
- EFT Ligation transfered colonies to a new plate and to liquid culture
- Electroporation of PAMS (Promotor, Sam5, Sam8)
[edit] Vanillin Receptor
- New PCR showed a better result for virA and geleclectrophoresis showed some result. The motherstrain was cut with DpnI to remove those residues, PCR-purification was performed and a restriction digest with PstI was done to see if restriction sites indeed were mutated
- virB ligation in K was again
