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Team:KULeuven/19 August 2009
From 2009.igem.org
Project progress
Progress of parts
[edit] Blue Light Receptor
[edit] Vanillin Production
- Miniprep sam8, sam5, ech, fcs plasmids in triple
- Nanodrop concentrations:
| Part | concentration (ng/μl) | 260/280 λ | |
|---|---|---|---|
| Sam8 A | 58,2 | 1,87 | |
| Sam8 B | 63,2 | 1,93 | |
| Sam5 A | 94,6 | 1,92 | |
| Sam5 B | 125,0 | 1,91 | |
| Sam5 C | 104,2 | 1,91 | |
| Ech A | 88,6 | 1,92 | |
| Ech B | 96,7 | 1,95 | |
| Ech C | 105,6 | 1,86 | |
| Fcs A | 84,4 | 1,88 | |
| Fcs B | 113,4 | 1,95 | |
| Fcs C | 90,3 | 1,92 |
- We took the plasmids with the lowest concentrations to perform the restriction test, described above. Afterwards, we ran the restriction products, together with uncut plasmids, on a gel.
- Calculations for restriction:
| Part | µl DNA | µl MQ | |
|---|---|---|---|
| Sam8 A | 8,7 | 7,3 | |
| Sam5 A | 5,3 | 10,7 | |
| Ech A | 5,6 | 10,4 | |
| Fcs A | 5,9 | 10,1 |
- Meanwhile ... one plated EF colony seemed to have grown. Made fluid culture of EF.
[edit] Vanillin Receptor
- Continued with ethanol precipitation for R. Electroporation and outplating were performed
