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Team:KULeuven/29 September 2009
From 2009.igem.org
Project progress
Progress of parts
[edit] Blue Light Receptor
we redid the experiment from 24/09 but only grew cultures at 25°C. we did split up the cultures into two groups, 16°c and 25°C and each group had some cultures that were lit and some dark.
[edit] Vanillin Production
- Nanodrop of the six cultures of PAMSCOEFT
| part | concentration | 260/280 | 260/230 |
|---|---|---|---|
| PAMSCOEFT1 | 147,4 | ||
| PAMSCOEFT2 | 170,8 | ||
| PAMSCOEFT3 | 177,3 | ||
| PAMSCOEFT4 | 167,5 | ||
| PAMSCOEFT5 | 150,4 | ||
| PAMSCOEFT6 | 166,2 |
- Restriction digest & Gel electrophorese
- All the 6 parts seemed to be self closed Terminator vectors.
- The result is probably due to an error in the ligation accompanied with the low probability of taking up a large vector with the size of PAMSCOEFT (10k bp)
[edit] Vanillin Receptor
- electropoation of virA was succesfull so it was ented
- virB was ented from the motherplate
[edit] Key/Lock/Anti-Key
- We finally received the lock and the antisense key from geneart.
- Electroporation and plating out the two components.
