"
Team:KULeuven/7 August 2009
From 2009.igem.org
Project progress
Progress of parts
[edit] Blue Light Receptor
[edit] Vanillin Production
Gel extraction of DNA from A1 (sam5) and A2 (sam8)
Results of the nanodrop:
| Part | concentration (ng/μl) | 260/280 λ | 260/230 λ |
|---|---|---|---|
| A1 (sam5) | 3,4 | 1,80 | 0,01 |
| A2 (sam8) | 3,1 | 2,66 | 0,02 |
- COMT was grown
- sam5 and sam8 were ligated
- ech and fcs were ligated
[edit] Vanillin Receptor
- Primers for mutagenesis have arrived
- We used the DNA parts from the second PCR to insert them into a TOPO-vector. The second PCR showed more results so that is why we used these fragments.
- LBmedium was made with X-gal to select cells afterwards.
- Plasmids were inserted in competent cells by heatshock and those cells were plated out on the X-gal medium.
