Team:KULeuven/9 September 2009

From 2009.igem.org

Project progress

Progress of parts

[edit] Blue Light Receptor

  1. FACS-ing the liquid cultures that were put under blue light on 08/09 gave no significant results.
  2. Motherplate for was made
  3. liquid cultures for checking the ligX were made.
  4. LC with were miniprepped, nanodropped, restriction digested and put on gel. The signals were good. The vector, pSB1A2, was cut from the gel and gel extracted (10ng/µl). This was ligated with (LigY).

Nanodrop:

Part concentration (ng/μl) 260/280 λ
(1) 141,8 1,94
(2) 139,5 1,99
(3) 177,5 1,95
(4) 141 1,95

[edit] Vanillin Production

  • Restriction digest on Terminator
  • Growth on SAMS plates (woohoo!)
  • Transferred 4 colonies from the SAMS plates (SAMS1, SAMS1 pellet, SAMS2, SAMS2 pellet) to a new plate
  • Gel electrophoresis of all the 8 EF restriction digests (2 per colony)
    • Cut and purified EF1 and EF3
part concentration 260/280 260/230
EF1 5,2 1,43
EF3 4,3 1,45

[edit] Vanillin Receptor

  • fluid cultures were made of rpoA, X+K and the motherplate of virG so we could miniprep this tomorrow
  • ligation of puc and A was electroporated
  • new ligation of X+K was tried out

[edit] Key/Lock/Anti-Key