Team:NYMU-Taipei/Project/Lab Note
From 2009.igem.org
Experiment of oscillator biobrick parts
1 | BBa_C0051 cI repressor from E. coli phage lambda (+LVA) |
2 | BBa_K116602 CII coding region from λ phage |
3 | BBa_C0040 tetracycline repressor from transposon Tn10 (+LVA) |
4 | BBa_C0074 penI repressor from Bacillus licheniformis (+LVA) |
5 | BBa_C0072 mnt repressor (strong) from Salmonella phage P22 (+LVA) |
6 | BBa_C0073 mnt repressor (weak) from Salmonella phage P22 (+LVA) |
7 | BBa_E0040 green fluorescent protein |
t | BBa_B0015 Terminator |
A | BBa_R0040 TetR repressible promoter |
P | BBa_R0074 Promoter (PenI regulated) |
L | BBa_R0010 promoter (lacI regulated) |
M | BBa_R0073 Promoter (Mnt regulated) |
C | BBa_R0051 promoter (lambda cI regulated) |
pCI | BBa_R1051 Promoter, Standard (lambda cI regulated) |
p22 | BBa_R0053 Promoter (p22 cII regulated) |
pLuxR | BBa_R0062 Promoter (luxR & HSL regulated -- lux pR) |
pLasR | BBa_R0079 Promoter (LasR & PAI regulated) |
E | BBa_K116603 pRE promoter from λ phage |
Experiment of oscillator biobrick parts (2)
| 2% agarose, 90V, 30min
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Experiment of oscillator biobrick parts (3)
| 2% agarose, 90V, 30min
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4: Inconclusive.
Experiment of oscillator biobrick parts (4)
| 2% agarose, 90V, 30min
The result for lanes 7-11 look weird, but it could be because of the gel. A rerun will be done. |
Experiment of oscillator biobrick parts (5)
| 2% agarose, 90V, 30min
|
Experiment of oscillator biobrick parts (6)
| 2% agarose, 90V, 30min
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Experiment of oscillator biobrick parts (7)
| 2% agarose, 90V, 30min
|
Experiment of oscillator biobrick parts (8)
| 2% agarose, 90V, 30min
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Experiment of oscillator biobrick parts (9) Gel Extraction
| 2% agarose, 90V, 30min
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Experiment of oscillator biobrick parts (10) (Gel Extraction)
| 2% agarose, 90V, 30min
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Experiment of oscillator biobrick parts (11)
| 2% agarose, 90V, 30min
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Experiment of oscillator biobrick parts (12)
| 2% agarose, 90V, 30min
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Experiment of oscillator biobrick parts (13) 2009/08/13
| 2% agarose, 90V, 38min
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Experiment of oscillator biobrick parts (14) (Gel Extraction) 2009/08/13
| 2% agarose, 90V, 36min
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Experiment of oscillator biobrick parts (15) 2009/08/15
| 2% agarose, 100V, 30min
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Experiment of oscillator biobrick parts (16) (Gel Extraction) 2009/08/15
| 2% agarose, 100V, 30min
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Experiment of oscillator biobrick parts (17) 2009/08/16
| 2% agarose, 100V, 30min
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Experiment of oscillator biobrick parts (18) (digestion) 2009/08/17
| 2% agarose, 100V, 30min
Comments
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Experiment of oscillator biobrick parts (19) (Gel Extraction) 2009/08/17
| 2% agarose, 100V, 30min
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Experiment of oscillator biobrick parts (20) 2009/08/18
| 2% agarose, 100V, 28min
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Experiment of oscillator biobrick parts (21) (Gel Extraction) 2009/08/18
| 2% agarose, 100V, 30min
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Experiment of oscillator biobrick parts (22) 2009/08/19
| 2% agarose, 100V, 28min
PCR of nhaA using this protocol worked. |
Experiment of oscillator biobrick parts (23) 2009/08/19
| 2% agarose, 100V, 28min
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Experiment of oscillator biobrick parts (24) 2009/08/20
| 2% agarose, 100V, 27min
Comments:
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Experiment of oscillator biobrick parts (25) 2009/08/21
| 2% agarose, 100V, 28min
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Experiment of oscillator biobrick parts (26) 2009/08/22
| 2% agarose, 100V, 28min
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Experiment of oscillator biobrick parts (27) 2009/08/23 colony PCR
| 2% agarose, 100V, 28min
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Experiment of oscillator biobrick parts (28) 2009/08/23 testing the reheating gel
| 2% agarose, 100V, 28min
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Experiment of oscillator biobrick parts (29) 2009/08/26
| 2% agarose, 100V, 29min
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Experiment of oscillator biobrick parts (29) 2009/08/27
| 2% agarose, 100V, 29min
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Experiment of oscillator biobrick parts (30) 2009/08/28
| 2% agarose, 100V, 29min
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Experiment of oscillator biobrick parts (31) 2009/08/31
| 2% agarose, 100V, 28min
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Experiment of oscillator biobrick parts (32) Gel Extraction 2009/08/31
| 2% agarose, 100V, 28min
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Experiment of oscillator biobrick parts (33) 2009/09/01
| 2% agarose, 100V, 29in
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Experiment of oscillator biobrick parts (34) Gel Extraction 2009/09/02
| 2% agarose, 100V, 28min
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Experiment of oscillator biobrick parts (35) 2009/09/03
| 2% agarose, 100V, 29min
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Experiment of oscillator biobrick parts (36) Gel Extraction 2009/09/03
| 2% agarose, 100V, 28min
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Experiment of oscillator biobrick parts (37) 2009/09/04
| 2% agarose, 100V, 29in
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Experiment of oscillator biobrick parts (38) Gel Extraction 2009/09/09
| 2% agarose, 100V, 28min
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Experiment of oscillator biobrick parts (39) 2009/09/11
| 2% agarose, 100V, 28in
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Experiment of oscillator biobrick parts (40) 2009/09/15
| 2% agarose, 100V, 29in
Comments:
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Experiment of oscillator biobrick parts (41) 2009/09/17
| 2% agarose, 100V, 29in
Comments: |
Experiment of oscillator biobrick parts (42) 2009/09/18
| 2% agarose, 100V, 28in
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Experiment of oscillator biobrick parts (43) 2009/09/22
| 2% agarose, 100V, 28in
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Experiment of oscillator biobrick parts (44) 2009/09/24
| 2% agarose, 100V, 28in
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Experiment of oscillator biobrick parts (45) (Gel Extraction) 2009/09/28
| 2% agarose, 100V, 30min
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Experiment of oscillator biobrick parts (46) 2009/10/17
| 2% agarose, 100V, 28in
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Experiment of oscillator biobrick parts (47) 2009/10/18
| 2% agarose, 100V, 30in
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