Team:Newcastle/Meetings/14 May 2009

From 2009.igem.org

IGEM Meeting 14 May 2009

Attendees: Neil Wipat, Hennifer Hallinan, Goksel Misirli, Hang Zhao, Daniel Swan

Action Points

  • Build a promoter library, use ClustalW for the alignment
  • Use microaray data to rank teh promoters based on their strenghths
  • Check if sigmaA is turned off during sporulation
  • Another approach for the stochastic swicth is using DNA flipping. Based on random DNA flipping we will have RFP wexpression or activated gerM expression. The promoter between the flipping region is turned to activate the right or the left side


[rfp RBS&CDS] - [lac promoter] - [invertase protein RBS&CDS] - [lac RBS&CDS] - [flipping region] - [promoter] - [flipping region] - [gerM RBS&CDS] - [gfp RBS&CDS]

stochastic switch gere would be :

[lac promoter] - [invertase protein RBS&CDS] - [lac RBS&CDS] - [flipping region] - [promoter] - [flipping region]

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