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Team:Stanford/NotebookPage/11 August 2009
From 2009.igem.org
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Daily Work
Goals
- Revise diagram breakdowns for Jerome
- Revise vBOL symbols
- Either research Th-17 to NO pathway or work on wiki
Project / Accomplishments
- Looks like transformations worked!!!! Yay! (1.1b and 2.1a)
- Mini-preps of SoxR/S cultures and pcr on 1.1b and 2.1a
- Need to sequence 1.2a
- Made revised constructions for Device 1
- New schematics for Diagrams
- vBOL symbols revised
Problems
- Simple schematic for Device 2....
Alternative Solutions
Events
- Meeting w/ Jerome for the presentation
- Show Naive CD4+ cells leading to T-cells?
- Testing line w/ filtering T-cells
- Numbers
- Rough percentage of amounts of T-cells (normal, non-recognizing, self-recognizing...)
- Why don't some self-recognizing t-cells undergo apoptosis?
- Circuit diagram
- Make text bigger, animation,...
- Take out IDO and dendritic cells?
- Shorter descriptions
- More visual support and animation
- Specifically on slides describing why we need device 2
- Device 2 parts breakdown- need separate promoter
- Visual- pyramid w/ age group and
- Immunology section- still too complicated
- More pictures
- Start w/ what the immune system "is" as a definition? Very general
- Stay away from capital letters
- Differentiate acquired and innate immune responses- mention vaccines!
- Talk more about acquired w/ animation (fun can be good)
- What is an antigen? What is an antibody?
- Scheme is nice- could be more simple
- Take away Tc cells
- Selective filtering- make a factory line and assembly line
- Define autoimmune diseases w/ respect to Self-recognizing t-cells
- Start w/ epidemiology then immune response, then back to IBD w/ respect to the immune system
- Mention system therapy vs. cure
- Showing the cause of inflammation itself.... b-bells, t-cells, and plasma cells (igE?)-
- Show transportation- lymph, vs blood, vs epithelial crossing
- Where are Naïve CD4+ cells? Needs to be in the place of activity!!!
- email Ariana or Professor Steinman
- Meeting w/ Christine for Stanford News at 2pm
- Interviews, vidoes, and revised article
- Looks good!
- FAB at 4:00
- Trp looks better, but not perfect yet...
- More modeling help needed?
- IBD Overview
- Be upfront of assumptions and parameters
- We assume it is t-cell ratios for inflammation
- Be upfront of assumptions and parameters
- Questions to answer
- Where are Naive CD4+ cells?
- What is the "cause" of IBD?
- Why don't medicines target t-cells?
- Also,
- Future- how can we biosynthesize 5MT?
- Trp looks better, but not perfect yet...
Comments
- Questions to be answered….
- Genetic component of the disease?
- Mouse model of IBD to test linear relationship of Th-17 and Treg cells
- Suicide device?
- Do people take RA for treatment?
- The acid form of Vitamin A, all-trans retinoic acid or ATRA, has been made into Tretinoin, which
- Do people take Il-6 for treatment?
- Other uses?
- Where are Naïve CD4+ cells? Needs to be in the place of activity!!!
- vBOL, are there names for the individual pictures
