Team:Todai-Tokyo/Protocols/PCR/programs

From 2009.igem.org

UT.png
Home The Team The Project Parts Submitted to the Registry Modeling Notebook Protocols Ethics




PCR Programs

Pfu Standard

  1. 95ºC 2 min
  2. 95ºC 30 sec
  3. 52-55ºC 30 sec （Depending on the Tm of the primers）
  4. 72.5ºC 15 sec × （# of kb of DNA to be amplified）
  5. 95ºC 30 sec Repeat 2-4 29 times
  6. 25ºC pause
Retrieved from "http://2009.igem.org/Team:Todai-Tokyo/Protocols/PCR/programs"