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Team:TorontoMaRSDiscovery/10 June 2009
From 2009.igem.org
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June 10, 2009
- Poured 10 Tet plates following procedure on June 5, 2009
- Due to problems with DNA ladder and restriction enzyme digest yesterday, procedures were followed again today with the following adjustments
- DNA was diluted and run on lanes 1-5 of gel:
- Lane 1 - 1X
- Lane 2 - 1/6X
- Lane 3 - 1/36X
- Lane 4 - 1/10X
- Lane 5 - 1/100X
- Restriction enzyme digest was repeated with an incubation time of 4 h at 37 degrees Celsius and heat shock at 80 degrees Celsius was done in hot water bath
- Results: Dilution did not work well (ladder could not be visualized). Digest appeared to work as 3 bands could be seen in last 2 lanes
- Adjustments for tomorrow:
- Spin down enzymes before using
- Overnight digest
- DNA was diluted and run on lanes 1-5 of gel:
