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From 2009.igem.org

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August 19, 2009

1. Miniprepped overnight cultures of Tet, K
2. Ran a gel of the ligations and the overnight digest
3. Started overnight culture of 4+5 colonies
4. Ligated Enc into CIPed C backbone
5. Started overnight K and Tet digestion
6. Poured Tet plates

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