Team:TorontoMaRSDiscovery/1 August 2009
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August 1, 2009
- Overnight encapsulin cultures 1 and 6 (randomly selected) and the negative control were miniprepped
- The rest of the encapsulin cultures were stocked with 20% glycerol
- 6 digestions were done: Enc1, Enc6, Enc -ve, BB5, BB7, and C
- 16ul of BB5 plasmid was used
- 500ng of plasmid were used for the others
- The digestions were run on a 1.3% agarose gel in TAE
- BB5 was confirmed and all other parts were correct as well
- Overnight ligation of 7+Enc in the PCR machine