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From 2009.igem.org

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May 21, 2009

1. Retrieved autoclaved ddH20, glycerol solution
2. Gel Electrophoresis (test run)
   • 1% gel: 0.5 g agar mixed with 50 ml of 1X TBE
   • 10X TBE diluted with 5 ml TBE + 45 ml ddH20 – did not use all 50 ml for 1 gel (gel will be too thick – make 2 instead)
   • Loading Dye: add glycerol solution to 25 mg bromophenol blue then add dH20 to 10 ml (to make 6X)
   • Running gel: match wells to black side, run at 120 mA
3. Visualize Gel in UV
   1. Turn power on
   2. Gel in machine face up
   3. Close door securely
   4. Turn white light on
   5. Adjust zoom, contrast, focus from black dial on top of machine
   6. Turn white light off (turns on UV)
   7. Press ‘live’ toggle – acq. Should be 0.4 sec.
   8. Print if desired or save on floppy disk
   9. Turn power off
   10. Dispose of gel in proper container
   11. Close door

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