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From 2009.igem.org

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August 28, 2009

1. Transfected 50ul of DH5 cells with 4+5(1) plasmid (2ul)
2. added 100ul of LB+glucose and plated all on a Tet plate
3. Mixed up LB+agar for pouring C plates on Monday (Aug 31)
4. Picked colonies growing on 4+5 plate and Enc plate and started overnight cultures

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