Team:TorontoMaRSDiscovery/4 October 2009

From 2009.igem.org


October 4, 2009

  1. Miniprepped, digested Tet and ran on gel
    • Tet was confirmed
  2. gel extracted EncY, Tet and got low yields
  3. Started overnight ligations
    • 1: Digested Tet + EncY + 5
    • 2: Gel extraced Tet + Digested EncY + 5
    • negative controls were Tet plasmid alone