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Team:TorontoMaRSDiscovery/5 June 2009
From 2009.igem.org
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June 5, 2009
- Tet plates made
- Recipe for 200 ml (approx. 10 plates):
- 2.2 g agar in 200 ml fresh LB
- Note: do not re-autoclave LB, it will caramelize!
- Recipe for 200 ml LB:
- a) 1 g yeast extract
- b) 2 g peptotryptone
- c) 2 g NaCl
- d) 200 ml water
- Recipe for 200 ml (approx. 10 plates):
- Autoclaved at 11:20 a.m., picked up at 1:15 p.m. and cooled in water bath at 37 degrees Celsius
- Added 200 microlitres tetracycline stock (from -20 freezer) to final concentration of 20 microgram/ml
- Swirl and poured into prepared, labeled plates
- Note: label on plates reads June 3, 2009 but should be June 5, 2009 (8 plates in total - either I overpoured or these plates are a bit larger than I was used to)
- Inverted and put in 37 degree incubator to dry
