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From 2009.igem.org

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August 7, 2009

1. Started overnight cultures of 1+2 sample 1,2,4
2. Retransformed BB1 using the iGEM 2007 Toronto team's protocol (heat shock for 90s) and plated on Amp plates
3. Plated DH5alpha cells as a control on plain LB plates
   • • This is thumotoga maritime genomic DNA for purpose of re-cloning
       
4. Microcentrifuge tubes 1 and 2 placed in -20 freezer

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