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From 2009.igem.org

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October 9, 2009

1. Miniprepped EncY overnight and digested with E,P
2. Ran digest on a gel
   • Enc part confirmed
3. Transfected Enc+5 ligations from Sunday/Monday into competent cells from Invitrogen
   • Used NEB Hight Efficiency Transformation Protocol C2987
     • did one 10-fold dilution
     • transformed 5ul of DNA
     • spread 100ul of cells on plate
     • shaker/incubator speed 232rpm

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