Team:TorontoMaRSDiscovery/9 October 2009
From 2009.igem.org
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October 9, 2009
- Miniprepped EncY overnight and digested with E,P
- Ran digest on a gel
- Enc part confirmed
- Transfected Enc+5 ligations from Sunday/Monday into competent cells from Invitrogen
- Used NEB Hight Efficiency Transformation Protocol C2987
- did one 10-fold dilution
- transformed 5ul of DNA
- spread 100ul of cells on plate
- shaker/incubator speed 232rpm
- Used NEB Hight Efficiency Transformation Protocol C2987