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From 2009.igem.org

AM

I came in this morning to culture the plates, and it seems as though we just squeaked by with a single colony on each. I cultured them with labels L1-10, L1-2, L2-10, L2-2.

Additionally, our D6 part (supposedly low copy count vector?) that we cultured from frozen stock yesterday grew in the shaker. It is red. I've left it there, as I don't have the time to miniprep or make frozen stock today.

Someone will need to come in tomorrow to see how the culturing went. If this fails, do we have enough to go straight from ligation to transformation again?

-JT

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