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Team:UNC Chapel Hill/3 August 2009
From 2009.igem.org
Today I checked the colony: it grew on both carb and kan, indicating that it was not the intended part. Discovered that PSTI (in FastDigest format from fermentas) must be deactivated by phenol:ethanol precipitation (this is why we didn't get any colonies). Experimented with electrocompetent cells: discovered that they arc at suggested voltage (voltage should be reduced to at least 2000V, 25uF to prevent arcing). -ML
